Safranal promoted farklılaşma ve Parkinson hastalığının hayvan modelinde dopaminerjik nöronların hayatta kalması.

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ContextSafranal (SAF) is verified to have potential effects in promoting nerve growth.ObjectivesThis study verifies the role of SAF in promoting dopaminergic neurons growth in vitro and in vivo.Material and methodsRat neural stem cells (NSC) were treated with 1, 20, or 100 ng/mL of SAF, and the expression levels of tyrosine hydroxylase (TH) and dopamine transporter (DAT) were assayed by flow cytometry and real-time PCR and the secretion of dopamine (DA) was assayed by ELISA. Then, 2 × 106 cells of SAF-treated NSC was administrated into PD rat models induced by 6-OHDA. The differentiation and survival of dopaminergic neurons was identified by fluorescence microscope and TH+ cells by immunostaining and DA secretion by ELISA at week 2 and week 4, respectively.ResultsAfter being treated with SAF at 20 and 100 ng/mL for 1 week, TH and DAT positive rates increased 1.4- and 1.7-fold (p ConclusionsSAF promoted the production of functional DA cells and alleviated PD, which may contribute to a new therapy for PD patients.

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