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Pramipexole (PPX) is prescribed for alleviating parkinson's disease symptoms like tremors and mobility issues, as well as addressing the discomfort in the legs and sudden leg movement impulses associated with restless legs syndrome. This study presents a practical, direct, non-extractive, and cost-effective analytical strategy for the quantitative determination of PPX. The method leverages fluorimetric detection, specifically monitoring the quenching effect on the emission intensity of Acid red 87, a fluorone dye. Complexation between PPX and Acid Red 87 occurs rapidly within a single reaction vessel under acidic conditions. A linear relationship was established between the observed decrease in Acid red 87 fluorescence intensity and PPX concentration, enabling the construction of a calibration curve spanning 50 to 1400 ng/mL. Critical variables influencing the PPX-dye complex system were systematically investigated and optimized. Comprehensive validation studies, conducted in accordance with International Council for Harmonisation (ICH) guidelines, confirmed the method's reliability. The validated approach was subsequently applied successfully to assay PPX in pharmaceutical tablets and to evaluate tablet content uniformity.
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