NeuralCipher Research overview
The text below is the source article’s original abstract under a verified reuse license. cc by
A quick, noninvasive method based on 31P NMR spectroscopy to measure the pH of samples containing phosphate buffer is presented. By taking advantage of all four phosphate species populated at acidic, mildly acidic, mildly alkaline and alkaline pH, the method is applicable over a wide range of pH values, from 1 to 13. This tool is most precise at pH values near the pKa values, namely from 5.8 to 8.0 and 10.6 to 13.0. To facilitate its use, a web application is presented that calculates the pH value directly from phosphate 31P chemical shift (ppm) when sodium or potassium is the cation: ( https://rmni.iqf.csic.es/software/31phnmr/ ). In addition, the potential of this approach to monitor reactions which generate or consume H+ is illustrated by following the hydrolysis of GTP catalyzed by Ras-like protein in brain 1a (Rab1a), an essential protein linked to Parkinson's disease and tuberculosis. The intrinsic GTPase rate of Rab1a is found to be 3.3 ± 0.8·10- 3 min at 37 °C, which places Rab1a amoung the Rabs with slow GTPase rates and the longer lived "active" GTP-bound states.
Scientific assessment
Research question
Assess the question within the original study’s scope.
Methods & sample
Check the source article for study design and sample details.
Key findings
An editorially approved findings summary is not yet published for this record.
Limitations
Generalizability, bias and clinical meaning require separate assessment.
Methods, samples and clinical conclusions are not inferred from bibliographic metadata.