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Abstract Purpose The aim of this study was to develop and validate StepAn, a novel low-cost software tool for automated gait analysis in rodents. We sought to determine whether this accessible system, which uses standard video from devices like smartphones, could sensitively detect motor deficits in a pharmacological model of Parkinsonian bradykinesia. Methods Wistar rats received a single intraperitoneal injection of saline (control), 0.15 mg/kg haloperidol, or 0.30 mg/kg haloperidol. One hour post-injection, gait was analyzed using both the traditional manual "Footprints" test and the StepAn software, which automates paw detection and stride length calculation from video recordings. Results Both analysis methods detected a significant reduction in average stride length in haloperidol-treated rats compared to controls (p
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