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Aims Parkinson’s disease is considered biologically a neuronal α-synuclein disease, largely ignoring the more widespread α-synuclein deposition that occurs in astrocytes, with the aim of this study to identify whether astrocytes accumulate small α-synuclein aggregates before or after neurons. Methods Fixed serial midbrain and striatal sections from M83 A53T transgenic mouse model of Parkinson’s disease and wild-type controls were histologically processed for multiplex labelling of α-synuclein and astrocytic markers and astrocyte quantitation performed on digital images using QuPath software. Results The density of astrocytes within the substantia nigra pars compacta was approximately 30% greater compared with other sampled regions ( P Conclusions These data suggest that small α-synuclein aggregates are normally cleared by astrocytes and that the substantia nigra pars compacta requires more astrocytic support for this function than other midbrain dopaminergic regions or the striatum. This adds another vulnerability factor to those already known for the substantia nigra with early deficits in clearance of small α-synuclein aggregates by astrocytes associated with an increased astrocytic reactivity in the A53T transgenic mouse model. Key Points The substantia nigra pars compacta contains a higher density of astrocytes than the ventral tegmental area or striatum, indicating a greater reliance on astrocytic function and a greater vulnerability to astrocyte dysfunction Small aggregates of α-synuclein were observed in wild-type midbrain and striatal astrocytes, indicating normal clearance of α-synuclein by these astrocytes Midbrain and striatal astrocytes from A53T transgenic astrocytes have more than double the number of astrocytes and more astrocytes containing α-synuclein aggregates which have a reactive morphological phenotype.
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