A novel alpha-synuclein K58N missense variant in a patient with Parkinson’s disease

Al-Azzani M, Weber S, Ramalingam N, Ramón M, Shvachiy L, Mestre G, Zech M, Sicking K, Ibáñez de Opakua A, Jayanthi V, Amaral L, Agarwal A, Chandran A, Chaves SR, Winkelmann J, Trenkwalder C, Schwager M, Pauli S, Dettmer U, Fernández CO, Lautenschläger J, Zweckstetter M, Busnadiego RF, Mollenhauer B, Outeiro TF.

· 2025-02-13

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Mutations and multiplications in the SNCA gene , encoding alpha-synuclein (aSyn), are associated with familial forms of Parkinson’s disease (PD). We report the identification of a novel SNCA missense mutation (NM_000345.4, cDNA 174G>C; protein K58N) in a PD patient using whole exome sequencing, and describe comprehensive molecular and cellular analysss of the effects of this novel mutation. The patient exhibited typical sporadic PD with early onset and a benign disease course. Biophysical studies revealed that the K58N substitution causes local structural effects, disrupts binding to membranes, and enhances aSyn in vitro aggregation. K58N aSyn produces fewer inclusions per cell, and fails to undergo condensate formation. The mutation increases the cytoplasmic distribution of the protein, and has minimal effect on the dynamic reversibility of serine-129 phosphorylation. In total, the identification of this novel mutation advances our understanding of aSyn biology and pathobiology.

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